| 货号 | 5259S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | PCM-1 (Q15) Antibody detects endogenous levels of total PCM-1 protein. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Percentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centrosomal satellites (3). PCM-1 is required for the assembly of several centrosomal proteins including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 228 |
Western blot analysis of extracts from RD, Jurkat, and A-204 cells using PCM-1 (Q15) Antibody. 使用PCM-1 (Q15) Antibody，免疫印迹（Western Blot）分析RD、Jurkat和A-204细胞中PCM-1蛋白水平。 | |
Flow cytometric analysis of A-204 cells, using PCM-1 (Q15) Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)比较，使用PCM-1 (Q15) Antibody (蓝色)进行流式细胞仪分析A-204细胞。 | |
Confocal immunofluorescent analysis of A204 cells using PCM-1 (Q15) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用PCM-1 (Q15) Antibody (绿色)标记，共聚焦免疫荧光分析A204细胞。使用DY-554 phalloidin(红色)标记微丝蛋白。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 |
