| 货号 | 2750S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | Oct-4 Antibody detects endogenous levels of total Oct-4 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:200) IF-IC (1:400) ChIP (1:25) |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Flow cytometric analysis of untreated NCCIT cells using Oct-4 Antibody (blue) compared to a nonspecific negative control antibody (red). 使用Oct-4抗体（蓝色）对未处理的NCCIT细胞进行流式细胞分析，使用非特异抗体做为阴性对照（红色）。 | |
Western blot analysis of NCCIT and NTERA2 cell extracts using Oct-4 Antibody. 使用 Oct-4抗体对NCCIT和NTERA2细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human seminoma (left) or normal testis (right), showing nuclear localization using Oct-4 Antibody. 使用Oct-4抗体对石蜡包埋的人精原细胞瘤（左）或正常精巢（右）进行免疫组化分析，此抗体显示目的蛋白位于细胞核。 | |
Confocal immunofluorescent analysis of NCCIT cells using Oct-4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). 使用 Oct-4抗体（绿色）和 β-Tubulin (9F3) Rabbit mAb兔单抗（Alexa Fluor® 555 偶联）#2116（红色）对NCCIT细胞进行激光共聚焦荧光分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Oct-4 Antibody #2750 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 NCCIT细胞与20μl Oct-4抗体或2μl Normal Rabbit IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人Oct-4启动子引物#4641，SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量出示相对于input染色质总量进行相对定量，Input染色质量设定值为1。 |
