| 货号 | 3580S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F/ChIP |
| 目标/特异性 | Nanog Antibody detects endogenous levels of total nanog protein. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:800) ChIP (1:25) |
| 供应商 | CST |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
Western blot analysis of extracts from NTERA2 and NCCIT cells using Nanog Antibody.使用Nanog抗体对NTERA2和NCCIT细胞提取物进行western blot分析。 | |
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Nanog Antibody.使用Nanog抗体对HeLa细胞（蓝色）和NTERA2细胞（绿色）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of NTERA2 cells (left) and HeLa cells (right) using Nanog Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用Nanog 抗体（绿色）对NTERA2（左）和HeLa细胞（右）进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 鬼笔环肽（红色）标记。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Nanog Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞与20μl Nanog抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人Oct-4启动子引物#4641，SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。 |
