| 货号 | 2786S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | INCENP (inner centromere protein antigens 135 kDa, 155 kDa) is a chromosomal passenger protein crucial for multiple events that mediate chromosome separation during mitosis (1). At prophase INCENP is associated with chromatin whereas during prometaphase and metaphase it translocates to the inner centromere (1). Depletion of INCENP results in aberrant chromosome alignment at the metaphase plate, incomplete chromosome separation, and disruption of proper spindle formation and cytokinesis (2). INCENP is part of the chromosomal passenger complex that also contains Aurora B, borealin and survivin (2). Aurora B and INCENP are mutually dependent on each other for proper localization (3), and in Drosophila cells and C.elegans embryos that lack INCENP or survivin, Aurora B cannot organize the kinetochores and the midbody (4,5). Phosphorylation on INCENP by CDK1 on Thr59 and Thr388 leads to the association of INCENP with Plk1, another important regulator of mitotic entry and exit (6). Interaction of INCENP with Plk1 is necessary for recruitment of Plk1 to the kinetochores, and the metaphase to anaphase transition (6). Interactions have also been reported between INCENP and heterochromatin protein 1α (HP1) (7) and β-tubulin (8).INCENP(内着丝粒蛋白抗原135 kDa，155 kDa)是一种染色体乘客蛋白，在有丝分裂过程中介导染色体分离的多重事件中至关重要(1)。在分裂前期，INCENP和染色质有关；而在前中期和中期，它转移到内着丝粒(1)。INCENP的减少可以引起异常的赤道板染色体排布、不完全染色体分离和适当的纺锤体形成以及胞质分裂的破坏(2)。INCENP是染色体乘客蛋白复合体的一部分，该复合体还包括Aurora B，borealin和survivin (2)。Aurora B 和 INCENP相互依赖以形成适当的定位(3)，在果蝇细胞和线虫胚胎中，缺少INCENP 或survivin,，Aurora B会导致无法组织着丝粒和中间体(4,5)。CDK1引起的INCENP第59位和388位苏氨酸磷酸化可导致INCENP与另一个重要有丝分裂调节子Plk1的联合作用(6)。INCENP与Plk1的相互作用对于招募Plk1至着丝粒以及中期到末期转换是必不可少的(6)。INCENP和异染色质蛋白1α (HP1) (7)和β-管蛋白(8)之间的相互作用亦有报道(8)。Kif11:4203/7265 |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts of Jurkat and MOLT4 cells using INCENP (A841) Antibody.Western blot方法检测Jurkat和MOLT4细胞提取物，使用的抗体为 INCENP (A841) Antibody。 | |
Confocal immunofluorescent analysis of mitotic HeLa cells during metaphase (left) or anaphase (right) using INCENP (A841) Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测有丝分裂中期（左侧）和后期（右侧）的HeLa细胞，使用的抗体为INCENP (A841) Antibody （绿色）和 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116（红色）。蓝色伪彩为DNA荧光染料（产品信息为DRAQ5® #4084 ）。 | |
Flow cytometric analysis of Jurkat cells, using INCENP (A841) Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术分析Jurkat细胞，使用的抗体为INCENP (A841) Antibody ，呈蓝色。非特异性阴性对照抗体作为对照，呈红色。 |
