| 货号 | 8023S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Fas (4C3) Mouse mAb recognizes endogenous levels of total Fas protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9). 受体Fas及其配体FasL的结合触发的细胞凋亡途径在免疫调节、发育和癌症的进程中起着重要作用（1,2）。 |
| 存放说明 | -20C |
| 计算分子量 | 40-50 |
Confocal immunofluorescent analysis of ACHN cells using Fas (4C3) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析ACHN细胞，使用抗体为 Fas (4C3) Mouse mAb鼠单抗（绿色）。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using Fas (4C3) Mouse mAb. Western blot 检测多种细胞系提取物，使用抗体为 Fas (4C3) Mouse mAb鼠单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with a human Fas construct, using Fas (4C3) Mouse mAb. Western blot方法检测293T细胞提取物：空质粒或人Fas转染，使用抗体为Fas (4C3) Mouse mAb鼠单抗。 | |
Flow cytometric analysis of HT-1080 cells using Fas (4C3) Mouse mAb (blue) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red). 流式细胞术检测HT-1080 细胞，使用抗体为 Fas (4C3) Mouse mAb（蓝色）鼠单抗，对比浓度匹配的Mouse (G3A1) mAb IgG1 Isotype Control #5415（红色）。 |
