| 货号 | 9837S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | ID3 (D16D10) Rabbit mAb recognizes endogenous levels of total ID3 protein. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain, but lack a basic DNA binding domain. Heterodimerization with bHLH transcription factors therefore functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID3 plays an important role in immune system development, where it has been shown to repress E2A-mediated differentiation of T cells (3). Studies in mouse models have shown that homozygous deletion of ID3 disrupts regulatory T cell differentation (4), and leads to development of γδ T cell lymphoma (5). Outside of the hematopoietic compartment, ID3 was shown to repress MyoD, implicating ID3 in TGFβ-mediated muscle repair (6). Similarly, research studies have shown that ID3 suppresses p21 in colon cancer cells, a function that is purported to promote the self-renewal capacity of putative cancer-initiating cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
Western blot analysis of extracts from various cell lines using ID3 (D16D10) Rabbit mAb. 使用ID3 (D16D10)Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HeLa (positive; left) and HCT-15 (negative; right) cells using ID3 (D16D10) Rabbit mAb. Actin filaments were labeled with DY-554 phalloidin (red). 使用ID3 (D16D10)Rabbit mAb（绿色）对HeLa（阳性；左）和HCT-15（阴性；右）细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554 phalloidin标记（红色）标记。 | |
Flow cytometric analysis of Raji cells using ID3 (D16D10) Rabbit mAb compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用ID3 (D16D10)Rabbit mAb对比着相同浓度的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红）对Raji细胞进行流式分析。 |
