| 货号 | 9034S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F/ChIP |
| 目标/特异性 | Ikaros (D10E5) Rabbit mAb recognizes endogenous levels of total Ikaros protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Ikaros family proteins, characterized by the presence of an N-terminal zinc finger DNA-binding domain and a C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. The Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers and heterodimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2). Ikaros, also known as Ikaros family zinc finger protein 1 (IKZF1) and Lymphoid transcription factor 1 (LYF-1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Research studies have shown that imbalanced expression of different Ikaros isoforms, as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 50-70 |
Confocal immunofluorescent analysis of Jurkat cells (positive; left) and HeLa cells (negative; right) using Ikaros (D10E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).对Jurkat细胞(阳性；左图）和HeLa细胞（阴性；右图）进行共聚焦分析，使用抗体是Ikaros (D10E5) Rabbit mAb (绿色)。 肌动蛋白微丝使用DY-554 phalloidin 标记（红色）。 | |
Western blot analysis of extracts from various cell lines using Ikaros (D10E5) Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). Western blot results confirm absence of detectable Ikaros protein in HeLa cells, in accordance with the immunofluorescence analysis.Western blot分析多种细胞系的细胞提取物，使用抗体是Ikaros (D10E5) Rabbit mAb (上图)或β-actin (D6A8) Rabbit mAb #8457 (下图)。Western blot结果证明了HeLa细胞中未检测到Ikaros，这与免疫荧光分析结果相一致。 | |
Flow cytometric analysis of Ramos cells using Ikaros (D10E5) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.对Ramos细胞进行流式细胞术分析，使用抗体是Ikaros (D10E5) Rabbit mAb (蓝色)，concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用来作为二抗。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 10 μl of Ikaros (D10E5) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
