| 货号 | 2787S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Lck (73A5) Rabbit mAb detects endogenous levels of total Lck protein. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 56 |
Flow cytometric analysis of Jurkat cells, using Lck (73A5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究Jurkat细胞，所用抗体为Lck (73A5) Rabbit mAb(蓝色) 与非特异性的阴性对照抗体(红色)。 | |
Western blot analysis of Jurkat cell extracts using Lck (73A5) Rabbit mAb.Western免疫印迹。用Lck (73A5) Rabbit mAb研究 Jurkat 细胞的细胞提取液。 | |
Confocal immunofluorescent analysis of a Jurkat cell nucleus using Lck (73A5) Rabbit mAb (red) and CD3 antibody (green). Blue=DRAQ5® #4084 fluorescent DNA dye.共聚焦免疫荧光分析Jurkat细胞核，所用抗体为Lck (73A5) Rabbit mAb(红色) 和 CD3 antibody (绿色)。Blue=DRAQ5® #4084 DNA 荧光染料。 |
