| 货号 | 8741S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 目标/特异性 | Met (L6E7) Mouse mAb recognizes endogenous levels of total Met protein. |
| 使用方法 | F(1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Confocal immunofluorescent analysis of HCT 116 (left) and T-47D (right) cells using MET (L6E7) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测HCT 116细胞 (左图)和T-47D (右图)，使用的抗体为MET (L6E7) Mouse mAb (绿色)。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084. | |
Flow cytometric analysis of unpermeabilized Ramos (red), T-47D (blue), HCT 116 (orange), and MKN-45 (green) cells using Met (L6E7) Mouse mAb.流式细胞术方法分析unpermeabilized Ramos细胞(红色), T-47D细胞(蓝色), HCT 116细胞(橘色)和 MKN-45细胞(绿色)，使用的抗体为Met (L6E7) Mouse mAb. |
