| 货号 | 13577S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:3200) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Confocal immunofluorescent analysis of H226 (positive, left) and MCF7 (negative, right) cells using OB-cadherin (16G5) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using OB-Cadherin (16G5) Mouse mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, MCF7 cells are negative for OB-cadherin. | |
Flow cytometric analysis of unpermeabilized LNCaP cells (blue) and unpermeabilized H460 cells (green) using OB-Cadherin (16G5) Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 fragment (Alexa Fluor 488 conjugate) #4408 was used as a secondary antibody. |
