| 货号 | 2839S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | SHIP2 (C76A7) Rabbit mAb detects endogenous levels of total SHIP2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Flow cytometric analysis of Jurkat (blue) and HeLa cells (green) using SHIP2 (C76A7) Rabbit mAb.流式细胞仪分析 Jurkat 细胞(蓝色)和HeLa 细胞 (绿色) ，所用抗体为SHIP2 (C76A7) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa (left) and Jurkat cells (right) using SHIP2 (C76A7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞 (左图) 和Jurkat细胞 (右图) ，所用抗体为SHIP2 (C76A7) Rabbit mAb (绿色). 肌动蛋白丝用DY-554 phalloidin 标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from OVCAR8, LN18 and HeLa cells using SHIP2 (C76A7) Rabbit mAb.Western免疫印迹。用SHIP2 (C76A7) Rabbit mAb研究OVCAR8, LN18 和HeLa 细胞的细胞提取液。 |
