| 货号 | 14323S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IP/IF-IC/F |
| 使用方法 | IP(1:100) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 44 |
Flow cytometric analysis of Jurkat cells (blue) and Raji cells (green) using Spi-B (D3C5E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of Raji (positive, left) and Jurkat (negative, right) cells using Spi-B (D3C5E) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of Spi-B from Raji cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Spi-B (D3C5E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Spi-B (D4V9S) Rabbit mAb #14377. | |
Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) using Spi-B (DC35E) Rabbit mAb co-stained with BDCA-2, CD19, and CD3. BDCA-2+ plasmacytoid dendritic cells and CD19+ B cells are positive for Spi-B, whereas CD3+ T cells are negative. Anti-rabbit IgG (H+L), (F(ab)2 ) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
