货号 | 4755S |
反应种属 | Human |
来源宿主 | Mouse |
应用 | IF-IC/F |
目标/特异性 | SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen. |
使用方法 | F(1:200) IF-IC (1:500) |
供应商 | CST |
背景 | SSEA4 (stage-specific embryonic antigen 4) is a glycolipid carbohydrate epitope expressed on the surface of human teratocarcinoma stem cells, human embryonic germ cells, and human embryonic stem cells (1). Expression of human SSEA4 decreases following differentiation of human embryonal carcinoma cells. Expression of the SSEA4 antigen is absent in murine pluripotent cells, but increases following differentiation (1,2). |
存放说明 | -20C |
计算分子量 | N/A |
Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用SSEA4 (MC813) 鼠源单克隆抗体(绿色)对NTERA2细胞进行激光共聚焦荧光分析。用DY-554鬼笔环肽标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料). | |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA cells (green) using SSEA4 (MC813) Mouse mAb.使用SSEA4 (MC813) 鼠源单克隆抗体对不可透化的Jurkat 细胞(蓝色)和不可透化的NTERA2细胞(绿色)进行流式细胞分析。 |