TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred)【科瑞奥博】

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TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred)

货号： 8184S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	8184S
反应种属	Human
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) recognizes endogenous levels of total TNF-α protein. TNF-α (D5G9) Rabbit mAb #6945 is more sensitive by western blot.
使用方法	WB(1:1000) IP (1:100) F (1:1600) IF-IC (1:400)

性能
供应商	CST
背景	TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
存放说明	-20C
计算分子量	18, 25

参考图片

Confocal immunofluorescent analysis of THP-1 cells, treated with TPA #4174 (80 nM, 16 hr) (left) or treated with TPA followed by LPS (1 μg/ml, 1 hr) (right), using TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析THP-1细胞，TPA #4174 (80 nM, 16 小时)处理 (左图) 或者 LPS (1 μg/ml, 1 小时) 后TPA 处理(右图)。使用抗体是TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (绿色)。肌动蛋白微丝通过DY-554 phalloidin标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Flow cytometric analysis of human peripheral blood lymphocytes, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin #9995 (2 μM, 4 hr), and Brefeldin A #9972 (1 μg/ml, last 3 hr of treatment) (right), using a CD3 antibody and TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred).流式细胞术分析人源外周血淋巴细胞，未处理(左图)或TPA #4174 (40 nM, 4小时), Ionomycin #9995 (2 μM, 4 小时), and Brefeldin A #9972 (1 μg/ml, 最后3小时)处理(右图)，使用抗体是CD3抗体和TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred)。

Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, overnight) and then untreated (-) or treated with LPS (1 μg/ml, 1 hr; +), using TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析THP-1细胞的细胞提取物，使用TPA #4174 (80 nM, 过夜)分化，其后未处理(-)或LPS (1 μg/ml, 1小时; +)处理，使用抗体是TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。