| 货号 | 85336S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IHC-P F |
| 目标/特异性 | CD8α (D8A8Y) Rabbit mAb recognizes endogenous levels of total CD8α protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). |
| 存放说明 | -20C |
| 计算分子量 | 29 |
| 参考文献 | 1 . Zamoyska, R. (1994) Immunity 1, 243-46. |
Immunohistochemical analysis of paraffin-embedded human lymphoma using CD8a (D8A8Y) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD8a (D8A8Y) Rabbit mAb. | |
Western blot analysis of extracts from MOLT-4 and PC-3 cells using CD8α (D8A8Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human Crohns diseased colon using CD8a (D8A8Y) Rabbit mAb. | |
Flow cytometric analysis of human whole blood using CD8α (D8A8Y) Rabbit mAb co-stained with CD4-PE, showing a distinct CD8α positive population with no cross reactivity to CD4 positive cells. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. |
