| 货号 | 13684T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | PD-L1 (E1L3N®) XP® Rabbit mAb recognizes endogenous levels of total PD-L1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 40-50 |
| 参考文献 | 1 . Dong, H. et al. (1999) Nat Med 5, 1365-9. 2 . Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34. 3 . Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16. 4 . Dong, H. et al. (2002) Nat Med 8, 793-800. 5 . Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5. 6 . Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64. 7 . Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37. 8 . Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41. 9 . Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73. 10 . Wimberly, H. et al. (2015) Cancer Immunol Res 3, 326-32. |
Western blot analysis of extracts from A549 cells, IFN-γ treated (100 ng/mL, 48 hr; +) or untreated (-), using PD-L1 (E1L3N™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from KARPAS-299, SUP-M2, and PC-3 cells using PD-L1 (E1L3N™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of untreated SUP-M2 cells using PD-L1 (E1L3N™) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded Karpas-299 (left) or PC-3 (right) cell pellets using PD-L1 (E1L3N™) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PD-L1 (E1L3N™) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human placenta using PD-L1 (E1L3N™) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). |
