| 货号 | 2717T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. |
| 使用方法 | WB(1:1000) F (1:1600) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 72 |
| 参考文献 | 1 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 2 . Iwashima, M. et al. (1994) Science 263, 1136-9. 3 . Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8. 4 . Chan, A.C. et al. (1995) EMBO J 14, 2499-508. 5 . Williams, B.L. et al. (1999) EMBO J 18, 1832-44. 6 . Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94. 7 . Wiestner, A. et al. (2003) Blood 101, 4944-51. 8 . Crespo, M. et al. (2003) N Engl J Med 348, 1764-75. |
Western blot analysis of extracts from Jurkat cells treated with hydrogen peroxide (2mM for 2 minutes) or with lambda phosphatase and extracts from Ramos cells treated with anti-human IgM (12 micrograms/ml for 2 minutes) using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb.Western免疫印迹。用Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb研究经过氧化氢(2mM, 2 min)或 lambda磷酸酶处理的Jurkat细胞的细胞抽提液和经anti-human IgM (12 micrograms/ml,2 min)处理的Ramos细胞的细胞提取液 | |
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM (green), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of Ramos cells, human IgM treated (left) or untreated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. |
