Keratin 20 (D9Z1Z) XP® Rabbit mAb【科瑞奥博】

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Keratin 20 (D9Z1Z) XP® Rabbit mAb

货号： 13063T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	13063T
同种亚型	Rabbit
反应种属	Human
来源宿主	Rabbit
应用	W/IHC-P/IF-IC/F
目标/特异性	Keratin 20 (D9Z1Z) XP^® Rabbit mAb recognizes endogenous levels of total keratin 20 protein.
使用方法	WB(1:1000) IHC-P (1:800) F (1:200) IF-IC (1:400)

性能
供应商	CST
灵敏度	Endogenous
背景	Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
存放说明	-20C
计算分子量	48
参考文献	1 . Moll, R. et al. (1982) Cell 31, 11-24. 2 . Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 3 . Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. 4 . Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. 5 . Zatloukal, K. et al. (2004) J Pathol 204, 367-76. 6 . Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. 7 . Moll, R. et al. (1992) Am J Pathol 140, 427-47. 8 . Karantza, V. (2011) Oncogene 30, 127-38.

参考图片

Confocal immunofluorescent analysis of HT-29 (left) and MCF7 (right) cells using Keratin 20 (D9Z1Z) XP^® Rabbit mAb (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye). 使用Keratin 20 (D9Z1Z) XP® Rabbit mAb (绿色)对HT-29细胞（左）和MCF7细胞（右）进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using Keratin 20 (D9Z1Z) XP^® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8547 (lower).使用Keratin 20 (D9Z1Z) XP® Rabbit mAb（上）或β-Actin (D6A8) Rabbit mAb #8547（下）对多种细胞提取物进行western blot分析。

Flow cytometric analysis of MCF7 (blue) and HT-29 (green) cells using Keratin 20 (D9Z1Z) XP^® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 647 Conjugate) #4414 was used as a secondary antibody.使用Keratin 20 (D9Z1Z) XP® Rabbit mAb对MCF7细胞（蓝色）和HT-29细胞（绿色）进行流式分析。Anti-rabbit IgG (H+L), F(ab)2Fragment (Alexa Fluor® 647 Conjugate) #4414被用作二抗。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Keratin 20 (D9Z1Z) XP^® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽(左)和抗原特异性肽段(右)存在的条件下，使用Keratin 20 (D9Z1Z) XP® Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded HT-29 (left) or MCF7 (right) cell pellets using Keratin 20 (D9Z1Z) XP^® Rabbit mAb.使用Keratin 20 (D9Z1Z) XP® Rabbit mAb对石蜡包埋的HT-29细胞（左）和MCF7细胞（右）进行免疫组化分析。