Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb
货号：
13674T 基本售价：
1550.0 元 规格：
20ul
产品信息
概述| 货号 | 13674T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb recognizes endogenous levels of total Toll-like receptor 9 protein. This antibody is predicted to recognize known full-length isoforms of Toll-like receptor 9, but not cleaved Toll-like receptor 9 protein. |
| 使用方法 | WB(1:1000)
IP (1:50)
F (1:200)
IF-IC (1:400) |
性能| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TLR9 is highly expressed in macrophages, dendritic cells, and B lymphocytes, and in humans has five isoforms generated by alternative splicing (15,16). TLR9 binds to unmethylated CpG motifs present on bacterial DNA and stimulates NF-κB via the MyD88 adaptor protein (17-19). In contrast to most TLR family members that are localized to the plasma membrane, TLR9 is an intracellular receptor localized to the ER in resting cells (20). Upon binding to CpG DNA, TLR9 is proteolytically processed and translocates to endo-lysosomal compartments where it binds MyD88, initiating downstream signaling (21-23). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Akira, S. (2003) J Biol Chem 278, 38105-8.
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9 . Horng, T. et al. (2001) Nat Immunol 2, 835-41.
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11 . Muzio, M. et al. (1997) Science 278, 1612-5.
12 . Wesche, H. et al. (1997) Immunity 7, 837-47.
13 . Suzuki, N. et al. (2002) Nature 416, 750-6.
14 . Irie, T. et al. (2000) FEBS Lett 467, 160-4.
15 . Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8.
16 . Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71.
17 . Hemmi, H. et al. (2000) Nature 408, 740-5.
18 . Bauer, S. et al. (2001) Proc Natl Acad Sci U S A 98, 9237-42.
19 . Takeshita, F. et al. (2001) J Immunol 167, 3555-8.
20 . Latz, E. et al. (2004) Nat Immunol 5, 190-8.
21 . Park, B. et al. (2008) Nat Immunol 9, 1407-14.
22 . Ewald, S.E. et al. (2008) Nature 456, 658-62.
23 . Sepulveda, F.E. et al. (2009) Immunity 31, 737-48.
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参考图片Confocal immunofluorescent analysis of RPMI 8226 (left) and THP-1 (right) cells using Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
Flow cytometric analysis of THP-1 cells (blue) and RPMI 8226 cells (green) using Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody. |
Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
Immunoprecipitation of Toll-like receptor 9 from Ramos cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 9 (D9M9H) XP® Rabbit mAb. |
