| 货号 | 14161T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | MUC1 (D9O8K) XP® Rabbit mAb detects endogenous levels of total MUC1 protein. Because this protein is heavily glycosylated, multiple bands or a smear may result from western blot analysis using this antibody. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 230, 400 |
| 参考文献 | 1 . Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231. 2 . Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64. 3 . Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24. 4 . Li, Y. et al. (2001) J Biol Chem 276, 6061-4. 5 . Ren, J. et al. (2002) J Biol Chem 277, 17616-22. 6 . Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47. 7 . Raina, D. et al. (2004) J Biol Chem 279, 20607-12. 8 . Wei, X. et al. (2005) Cancer Cell 7, 167-78. |
Confocal immunofluorescent analysis of ZR-75-1 (positive, left) and HCT 116 (negative, right) cells using MUC1 (D9O8K) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HCT 116 (blue) and ZR-75-1 (green) using MUC1 (D9O8K) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from ZR-75-1, MCF 10A, and HCT 116 cells using MUC1 (D9O8K) XP® Rabbit mAb (upper) and β-Tubulin Antibody #2146 (lower). |
