| 货号 | 14803T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | Integrin β4 (D8P6C) XP® Rabbit mAb recognizes endogenous levels of total integrin β4 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 210 |
| 参考文献 | 1 . Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71. 2 . Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100. 3 . van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98. 4 . Schaapveld, R.Q. et al. (1998) J Cell Biol 142, 271-84. 5 . Litjens, S.H. et al. (2006) Trends Cell Biol 16, 376-83. 6 . Guo, W. et al. (2006) Cell 126, 489-502. 7 . Bertotti, A. et al. (2006) J Cell Biol 175, 993-1003. |
Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). | |
Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of A-204 cells (blue) and A-431 cells (green) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
