| 货号 | MCA5960GA |
| 克隆号 | 2B9 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | F, IF, IP, WB |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Flow cytometric staining of human S1P1 on CHO cells stably transfected with rHuS1P1-GFP (red) or CHO-K1 cells as a negative control (black) detected by GFP fluorescence [d] or with Mouse anti Human Sphingosine 1-phosphate receptor 1 [d] (MCA3960GA) | |
Immunofluorescence staining of human S1P1 with Mouse anti Human Sphingosine 1-phosphate receptor 1 on Huvec cells (MCA5960GA) [bottom] or with no primary antibody as a negative control [top)], followed by an anti Mouse IgG secondary conjugated to AlexaFluor488® | |
Immunofluorescent of CHO cells stably transfected with human S1P1-GFP or CHO-K1 cells using GFP fluorescence [a,b,i,j] or using Mouse anti Human Sphingosine 1-phosphate receptor 1 (MCA5960GA) [c,d], no primary control [k,l]. Merged images [g,h,o,p] demonstrate co-localization of GFP-S1P1 [h] | |
Immunoprecipitation of human S1P1 from CHO cells stably transfected with human S1P1-GFP lane 1: CH0-S1P1-GFP Lane 2: Control with no primary antibody Lane 3: CHO-S1P1-GFP solubized with NP-40 Lane 4: Immunoprecipitated sample using Mouse anti Human Sphingosine 1-phosphate receptor 1(MCA5960GA). Samples were probed using a Rabbit anti GFP coupled to Horseradish peroxidase and visualized using chemiluminescence | |
Western blot analysis of human S1P1 expression on CHO and Huvec cell lines using Mouse anti Human Sphingosine 1-phosphate receptor 1 (MCA5960GA) Lane 1: CHO-K1 cell membranes (negative control) Lane 2: CHO-S1P1-GFP stable transfectant Lane 3: Huvec cell membranes The GFP-S1P1 transfectant migrates at an apparent MW of ~70kDa whilst the native S1P1 expressed on HUVEC cells migrates at an apparent MW of ~43kDa |
