| 货号 | IC13351A-100 |
| 别名 | Cytokine ML-1; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24 | 全称 | Interleukin 17F |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human IL-17F in direct ELISAs. Does not cross-react with recombinant human IL‑17A, B, C, D, or E. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 112744 (Human); 257630 (Mouse); 301291 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-17F Arg31-Gln163 Accession # Q96PD4 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑17F in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL‑17F APC‑conjugated Monoclonal Antibody (Catalog # IC13351A) and Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
