Human TLR3 Allophycocyanin Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Human TLR3 Allophycocyanin Affinity Purified PAb (100 TESTS)

货号： IC1487A 基本售价： 4808.5 元规格： 100 Tests

产品信息

概述
货号	IC1487A
别名	CD283 antigen; CD283; toll-like receptor 3
全称	Toll-like Receptor 3
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human TLR3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR4 and recombinant mouse TLR6 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7098 (Human); 142980 (Mouse); 364594 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TLR3 Lys27-Ser711 Accession # O15455
生物活性	Human
标记	Allophycocyanin
背景	Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 Receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8-11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11-13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13-15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588. Choe, J. et al. (2005) Science 309:581. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133. Alexopoulou, L. et al. (2001) Nature 413:732. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542. Rudd, B.D. et al. (2005) J. Virol. 79:3350. Farina, C. et al. (2005) J. Neuroimmunol. 159:12. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502. Wang, T. et al. (2004) Nat. Med. 10:1366.

参考图片

Detection of TLR3 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human TLR3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1487A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.