| 货号 | FAB614A |
| 别名 | cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 receptor subunit alpha-2; IL13BP; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13RA2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein | 全称 | Interleukin 13 Receptor alpha 2 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3598 (Human); 16165 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2 Cys22-Leu342 Accession # Q14627 |
| 生物活性 | Human |
| 标记 | Allophycocyanin |
| 背景 | Interleukin-13 Receptor alpha 2 (IL-13 R alpha 2), alsoCD213 alpha 2, is a 55-57 kDa member of the type I cytokine receptor family ofmolecules. Human IL-13 R alpha 2 is a 354amino acid (aa) type I transmembrane glycoprotein that contains a 317 aaextracellular domain (ECD) characterized by three fibronectin type III domains(aa 27-343). It is expressed by alimited number of cell types, principally those associated with the respiratorysystem. These cells include fibroblasts,type II alveolar and visceral smooth muscle cells, pseudostratified ciliatedepithelium, goblet cells and possibly monocytes. Notably, IL-13 R alpha 2 is often found to beintracellular, rather than plasma membrane expressed, and this may reflectstorage deposits that can be quickly accessed when needed. IL-13 R alpha 2 is one of two IL-13Rs that bind IL-13. The principal, but low affinity receptor forIL-13 is a 65 kDa transmembrane protein termed IL-13 R alpha 1. Following binding to IL-13, this proteincomplexes with an adjacent IL-4 R alpha receptor on the cell surface to form a highaffinity, functional receptor signaling complex for IL-13. Within this system, IL-13 R alpha 2 has beendescribed as a "decoy receptor" that binds to circulating IL-13, rendering itunavailable for IL-13 R alpha 1 binding. Supporting this view is the fact that there does not appear to be anycanonical signaling motif in IL-13 R alpha 2s short cytoplasmic tail, and the fact thatsoluble IL-13 R alpha 2will bind to IL-13 and neutralize its effects on target cells in culture. The latter point is of particular importancewhen it is recognized that IL-13 R alpha 2 has a naturally circulating soluble spliceform in mouse. Studies in human,however, suggest a more complicated situation. First, it is unclear if human actually has circulating IL-13 R alpha 2. Unlike in mouse, there is no potential alternativesplice form in human that could generate a soluble isoform. And while MMP-8 has been identified as apotential mediator of IL-13 R alpha 2 proteolytic cleavage, such activity would appearto generate rather small quantities of soluble IL-13 R alpha 2. As a transmembrane protein, IL-13 R alpha 2 is suggestedto interact with IL-4 R alpha, and this likely will result in a downregulationof IL-13 activity. Thus, IL-13 R alpha 2, whilenot a decoy receptor, could act as a negative modulator of IL-13 signaling. On the other hand, the cytoplasmic tail ofIL-13 R alpha 2is now believed to stimulate AP-1 activity, and the IL-13:IL-13 R alpha 2transmembrane complex has been identified as a component of a larger signalingreceptor complex for the GH-18 (Glycosyl Hydrolase 18) family memberYKL40/Chi3L1. As such, IL-13 R alpha 2 islikely to exhibit multiple actions in a system and species-specific manner. Over aa 27-342, human and mouse IL-13 R alpha 2 share64% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑13 R alpha 2 in A375 Human Cell Line by Flow Cytometry. A375 human melanoma cell line was stained with Goat Anti-Human IL‑13 R alpha 2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB614A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins. |
