Hu Caspase-7 Pure B94-1 100ug
货号：
556541 基本售价：
5035.0 元 规格：
100ug
产品信息
概述| 货号 | 556541 |
| 描述 | Caspase-7 is a member of a family of cysteine proteases which play a critical role in the induction of apoptosis. Caspase-7 is structurally and functionally most similar to caspase-3. These proteases are expressed as proenzymes which are processed into large and small subunits that associate to form the active enzyme. Caspase-7 may also exist as alternative splice forms, i.e., Mch3 α and Mch3 β . A novel interaction between Mch3 α and the 17 kDa subunit of caspase-3 has been demonstrated in vitro , producing a heteromeric enzyme which is proteolytically active. Active caspase-7 can move to the mitochondrial fraction of cells undergoing Fas-mediated apoptosis, whereas active caspase-3 is primarily cytosolic. Caspase-7 is activated by caspase-31 and by granzyme B. Active caspase-7 can cleave the nuclear substrate PARP1 as well as the sterol regulatory element-binding protein 1 (SREBP-1). Caspase-7 migrates at a molecular weight of 35 kDa in SDS/PAGE. Clone B94-1 recognizes the 35 kDa and 17 kDa form human caspase-7. The specificity of the antibody was verified by immunoprecipitation and western blot analysis of active, recombinant human caspase-7 and of endogenous caspase-7 in cell lysates. A synthetic peptide corresponding to amino acids 25-42 of human caspase-7 was used as immunogen. |
| 别名 | Mch3 |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IP |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human Capase-7 aa. 25-42 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
