Hu Caspase-7 Pure 8-1-47 50ug
货号：
551236 基本售价：
3312.0 元 规格：
50ug
产品信息
概述| 货号 | 551236 |
| 描述 | The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspases are synthesized as inactive proenzymes containing three domains, that are processed into large and small subunits that associate to form the active enzyme . Processing can occur in apoptotic cells by either transactivation, self-proteolysis, or cleavage by another protease. While caspases share a common structure, there are some differences, such as the preferred substrate specificity. These sequence differences in specificity, as well as the size of the NH2 -terminal prodomains can be used to catagorize the caspases into functional groups including, apoptotic initiators (long prodomains), apoptotic executioners (short prodomains), and cytokine processors. Caspase-7, along with caspase-3 and -6 are members of the apoptotic executioners group containing short prodomains; caspase-7 is structurally and functionally most similar to caspase-3. Upon induction of apoptosis, pro-caspase-7 (35 kDa) is first converted to a 32 kDa intermediate, which is further processed into active subunits consisting of 20 kDa and 11 kDa forms (Swiss-Prot P55210). Active caspase-7 has been shown to cleave the nuclear substrate PARP as well as the sterol regulatory element-binding protein 1 (SREBP-1). In cells undergoing Fas-mediated apoptosis in vivo , active caspase-7 has been shown to translocate from the cytosol to the mitochondrial and microsomal fractions, whereas caspase-3 remains cytosolic. This data supports the hypothesis that similar apoptotic executioners cleave distinct substrates in different cellular compartments. The antibody recognizes human caspase-7. Full-length recombinant human caspase-7 protein was used as immunogen. The antibodies are routinely tested by western blot and immunoprecipitation analysis using Jurkat T cells (please refer to Table I for what forms of caspase-7 are identified in a particular application). |
| 别名 | Mch3 |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB, IP |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human Caspase-7 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
