货号 | 4418S |
描述 | Anti-rat IgG (H+L) was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. |
反应种属 | rat |
来源宿主 | Goat |
应用 | IF-IC/F |
使用方法 | IF-IC () |
供应商 | CST |
标记 | Alexa Fluor 647 |
背景 | Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence. |
Flow cytometric analysis of untreated HeLa cells using HSP70 (6B3) Rat mAb #4873 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (green) and compared to a nonspecific negative control antibody (red).流式细胞术分析HeLa细胞,所用抗体为HSP70 (6B3) Rat mAb #4873 ,检测抗体为Anti-Rat IgG (H+L) (Alexa Fluor ® 647 Conjugate) (绿色) ,与非特异性阴性对照抗体(红色)作对比。 | |
Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb #4736 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (blue) and COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光分析HeLa细胞,所用抗体为ORC2 (3G6) Rat mAb #4736 检测抗体为Anti-Rat IgG (H+L), (Alexa Fluor ® 647 Conjugate) (蓝色) ,和COX IV (3E11) Rabbit mAb #4850,检测抗体为Anti-Rabbit IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 488 Conjugate) #4412 (绿色) 。肌动蛋白丝采用DY-554 phalloidin (红色)标记。 |