货号 | 8889S |
描述 | Anti-rabbit IgG (H+L), F(ab)2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab)2 fragment results in less non-specific binding to cells through Fc receptors. |
反应种属 | rabbit |
来源宿主 | Goat |
应用 | IF-IC/F |
使用方法 | IF-IC () |
供应商 | CST |
标记 | Alexa Fluor 594 |
背景 | Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence. |
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞,使用COX IV (3E11) Rabbit mAb #4850检测Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 594 Conjugate)(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 detected with Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 594 Conjugate).流式细胞检测分析Jurkat细胞,未处理(绿色)或用LY294002 #9901、Wortmannin #9951和U0126 #9903(蓝色)处理,使用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060检测Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 594 Conjugate)。 |