Anti-rabbit IgG (H+L)F(ab)2 Fragment (Alexa Fluor® 488 Conjugate)【科瑞奥博】

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Anti-rabbit IgG (H+L)F(ab)2 Fragment (Alexa Fluor® 488 Conjugate)

货号： 4412S 基本售价： 1798.0 元规格： 250ul

产品信息

概述
货号	4412S
描述	Anti-Rabbit IgG (H+L) F(ab)2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab)2 fragment results in less non-specific binding to cells through Fc receptors.
反应种属	rabbit
来源宿主	Goat
应用	IF-IC/F/HCA
使用方法	F () IF-IC ()

性能
供应商	CST
标记	Alexa Fluor 488
背景	Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

参考图片

Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody #3978 detected with Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析P19细胞，所用抗体为LIN28A (A177) Antibody #3978检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) (绿色)。肌动蛋白丝采用DY-554 phalloidin (红色)，蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084。

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 detected with Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) and compared to a nonspecific negative control antibody (red).流式细胞术分析Jurkat细胞，绿色是未处理组，蓝色为采用LY294002 #9901, Wortmannin #9951 和 U0126 #9903 (blue)处理组。所用抗体为Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060，检测抗体为Anti-Rabbit IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 488 Conjugate) (绿色) ，与非特异性阴性对照抗体（红色）作对比。

High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated control was observed. When using phospho-MAPKAPK-2 as a measurement, the IC50 of this compound was 92.5 mM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate).高通量分析HeLa细胞，该细胞暴露于不同浓度的十字孢碱3个小时。与未处理的对照组相比较，随着十字孢碱浓度的增加phospho-MAPKAPK-2信号显著降低（约2.5倍）。当采用 phospho-MAPKAPK-2检测时，IC50为92.5mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate)。