| 货号 | 4414S |
| 描述 | Anti-rabbit IgG (H+L) F(ab)2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab)2 fragment results in less non-specific binding to cells through Fc receptors. |
| 反应种属 | rabbit |
| 来源宿主 | Goat |
| 应用 | IF-IC/F/HCA |
| 使用方法 | IF-IC () |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence. |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9906 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 detected with Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) compared to a nonspecific negative control antibody (red).流式细胞术分析Jurkat细胞，绿色为未处理组，蓝色为采用LY294002 #9901, wortmannin #9951 和U0126 #9906 处理组，所用抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb #4858，检测用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate) ，与非特异性阴性对照抗体作比较（红色）。 | |
Confocal immunofluorescent analysis of mitotic HeLa cells using β-Tubulin (9F3) Rabbit mAb #2128 detected with Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) (blue) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 (red).激光共聚焦免疫荧光分析有丝分裂的HeLa细胞，所用抗体为β-Tubulin (9F3) Rabbit mAb #2128检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647Conjugate) (蓝色，上图)和Phospho-Histone H3 (Ser10) (D2C8) XP™ Rabbit mAb (Alexa Fluor ® 555 Conjugate) #3475 (红色). 。 | |
High content analysis of A549 cells exposed to varying concentrations of LY294002 (#9901) for 3 hrs, followed by 100 ng/mL EGF for 20 minutes. With increasing concentrations of LY294002, a significant decrease (~5 fold) in phospho-S6 Ribosomal Protein (Ser235/236) signal as compared to the uninhibited control was observed. When using phospho-S6 as a measurement, the IC50 of this compound was 3.06 μM. Data were generated on the Acumen® HCS platform using Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate).图 高通量分析A549细胞，该细胞暴露于不同浓度的LY294002 #9901 3个小时。然后用100ng/mlde EGF 处理20分钟。与未抑制组相比较，随着LY294002 #9901浓度的增加phospho-S6 Ribosomal Protein (Ser235/236)信号显著降低（约5倍）。当phospho-S6 Ribosomal Protein 检测时，IC50为3.06mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate)。 |
