| 货号 | 4851S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858. |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
Flow cytometric analysis of K562 cells, untreated (green) or STI571-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb兔单抗 (Alexa Fluor® 647接合)标记，流式细胞术分析K562细胞，细胞分为未处理组（绿色）和STI571处理组（蓝色）。 | |
Confocal immunofluorescent analysis of HeLa cells treated with 20% serum (left) or with Rapamycin, Wortmannin and U0126 inhibitors (right) using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb 兔单抗(Alexa Fluor® 647接合) #4851，共聚焦免疫荧光观察磷酸化S6核糖体蛋白在HeLa细胞中定位，分为20%血清处理组（左图）和Rapamycin, Wortmannin和U0126抑制剂处理组（右图）。 |
