| 货号 | 4854S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856. |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-F/IF-IC/F |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. |
| 使用方法 | F(1:50) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (#4854). 使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (#4854)标记，流式细胞仪分析Jurkat细胞，细胞分为未处理组（绿色）和LY294002，Wortmannin和U0126-treated处理组（蓝色）。 | |
Confocal immunofluorescent analysis of two Gefinitib (Iressa) treated non-small cell lung cancer cell lines. HCC827 cells have the E746_A750 deletion in exon 19 of the EGFR gene, and are highly sensitive to Gefitinib. H1975 cells have the (T790M) mutation that confers Gefitinib-resistance. Both cell lines were treated and then double-labeled with Phospho-S6 Ribosomal Protein Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 and Phospho-Tyrosine Mouse mAb (Alexa Fluor® 647 Conjugate) #9415. Untreated HCC827 (A) and H1975 (C) cells show bright phospho-S6 (green) and phospho-tyrosine (red pseudocolor) label. Phospho-S6 and phospho-tyrosine signals dramatically decrease following Gefitinib treatment in HCC827 cells (B), with little or no change in the Gefitinib-resistant H1975 cells (D). 共聚焦免疫荧光观分析两种 Gefinitib (Iressa) 处理的非小细胞肺癌细胞系。HCC827细胞系在EGFR第19外显子基因存在E746_A750突变，且对Gefitinib高度敏感。 H1975细胞系有 (T790M)突变，且该突变赋予Gefitinib耐药。两种细胞系都被Gefinitib (Iressa) 处理后，然后使用Phospho-S6 Ribosomal Protein Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854和Phospho-Tyrosine Mouse mAb (Alexa Fluor® 647 Conjugate) #9415进行双标。Gefinitib未处理的HCC827(A)和H1975 (C)细胞系显示明亮的phospho-S6 (绿色)和phospho-tyrosine (红色)标记。在Gefinitib处理HCC827细胞系(B)中Phospho-S6 和phospho-tyrosine信号明显减少，而在Gefitinib耐药的H1975细胞系中很少或没有改变(D)。 | |
Confocal immunofluorescent analysis of rat brain showing the hippocampus (left) and striatum (right) labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green), EGR1 Antibody (red) #4152, and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue) following ischemia with 30 minute reperfusion. 使用Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate)（绿色），EGR1 Antibody (red) #4152和Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue)标记，共聚焦免疫荧光分析大鼠缺血再灌注损伤30分钟后大脑海马组织（左图）和纹状体组织（右图）。 |
