| 货号 | 9177S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F/ChIP |
| 目标/特异性 | Phospho-Stat1 (Ser727) Antibody detects endogenous levels of Stat1α only when phosphorylated at Ser727. This site is deleted in Stat1β. This antibody does not significantly cross-react with the corresponding phosphorylated residues of other Stat proteins. |
| 使用方法 | WB(1:1000) IF-IC (1:100) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 计算分子量 | 91 |
Western blot analysis of extracts from 293 (human), NIH/3T3 (mouse), and C6 (rat) cells, untreated or UV-stimulated, using Phospho-Stat1 (Ser727) Antibody | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left), IFN-γ treated (center) or IFN-γ and phosphatase treated (right), labeled with Phospho-Stat1 (Ser727) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). | |
Flow cytometric analysis of Hela cells, phosphatase-treated (red), untreated (blue) or UV-treated (green), using Phospho-Stat1 (Ser727) Antibody. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 20 μl of Phospho-Stat1 (Ser727) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
