Phospho-RelB (Ser552) Antibody【科瑞奥博】

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Phospho-RelB (Ser552) Antibody

货号： 4999S 基本售价： 3980.0 元规格： 100ul

产品信息

概述
货号	4999S
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	Phospho-RelB (Ser552) Antibody detects endogenous levels of RelB only when phosphorylated at Ser552.
使用方法	WB(1:1000) IP (1:100) F (1:400) IF-IC (1:200)

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	70

参考图片

Flow cytometric analysis of Raji cells, untreated (blue) or TPA-treated (green), using Phospho-RelB (Ser552) Antibody.流式细胞仪分析未经处理(蓝色) 或经TPA (绿色)处理的Raji细胞。所用抗体为 Phospho-RelB (Ser552) Antibody。

Confocal immunofluorescent analysis of Raji cells, serum-starved (left), treated with TPA (Phorbol-12-Myristate-13-Acetate) #9905 (center) or λ phosphatase-treated (right), using Phospho-RelB (Ser552) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析Raji 细胞，经血清饥饿(左图), TPA (Phorbol-12-Myristate-13-Acetate) #9905 处理(中间图)或 λ phosphatase处理 (右图)，所用抗体为Phospho-RelB (Ser552) Antibody (绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)

Western blot analysis of extracts from A20 and CTTL2 cells, untreated or treated with TPA #4174 (200nM, 30 minutes) alone or with λ phosphatase, using Phospho-RelB (Ser552) Antibody.Western免疫印迹。用 Phospho-RelB (Ser552) Antibody研究未经处理的和经TPA #4174 (200nM, 30 min) 或经TPA #4174 (200nM, 30 min)与 λ phosphatase共处理的 A20 和 CTTL2 细胞的细胞提取液。