| 货号 | 9713S |
| 反应种属 | Human/Mouse/Hamster/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC/F |
| 目标/特异性 | Phospho-Histone H3 (Ser28) Antibody detects endogenous levels of histone H3 only when phosphorylated at Ser28. This antibody does not cross-react with other phosphorylated histones, including phospho-histone H3 (Ser10). |
| 使用方法 | WB(1:1000) IP (1:25) F (1:400) IF-F (1:400) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of lysates from CHO and HeLa cells either untreated or synchronized in metaphase by treatment with 0.1 mg/ml nocodazole for 4 h, followed by isolation of metaphase cells by mitotic shake-off. Blots were probed with Phospho-Histone H3 (Ser28) Antibody #9713 (upper) or Histone H3 Antibody #9715 (lower). 使用Phospho-Histone H3 (Ser28) Antibody #9713 (上图)或Histone H3 Antibody #9715 (下图)，免疫印迹(Western blot)分析CHO和HeLa细胞中Phospho-Histone H3 (Ser28)和Histone H3蛋白水平，未处理或有丝分裂中期使用0.1 mg/ml nocodazole处理4小时和随后通过有丝分裂摆脱法分离有丝分裂中期细胞。 | |
Confocal immunofluorescent analysis of C2C12 cells using Phospho-Histone H3 (Ser28) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Phospho-Histone H3 (Ser28) Antibody (绿色)标记，共聚焦免疫荧光分析C2C12细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Confocal immunofluorescent analysis of postnatal day 1 rat brain using Phospho-Histone H3 (Ser28) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Phospho-Histone H3 (Ser28) Antibody (绿色)，共聚焦免疫荧光分析出生后1天大鼠大脑组织。蓝色= DRAQ5™ (DNA荧光染料)。 | |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser28) Antibody versus propidium iodide (DNA content). The box indicates phospho-Histone H3 positive cells. |
