| 货号 | 4923S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. The antibody cross-reacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15-20 |
Flow cytometric analysis of COS cells, untreated (blue) or λ phosphatase-treated (green), using Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb compared to a nonspecific negative control antibody (red). 与非特异的阴性对照抗体（红色）比较，使用Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb兔单抗，流式细胞术分析COS细胞，分为未处理组 (蓝色)和λ磷酸酶处理组(绿色)。 | |
Western blot analysis of extracts from COS cells, treated with λ phosphatase or 20% FBS as indicated, using Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb (upper), Phospho-4E-BP1 (Thr37/46) Antibody #9459 (middle), and 4E-BP1 Antibody #9452 (lower). 使用Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb兔单抗 (上图)、Phospho-4E-BP1 (Thr37/46) Antibody #9459 (中间图)和4E-BP1 Antibody #9452 (下图)，免疫印迹（Western Blot）分析COS细胞系裂解物，按如图所示加入λ磷酸酶或20%FBS处理。 | |
Confocal immunofluorescent analysis of 293 shRNA Scramble cells either serum starved (far left); serum starved and treated with U0126 #9903 (10 μM, 2 hr), LY294002 #9901 (50 μM, 2 hr), and Rapamycin #9904 (10 nM, 2 hr) (center, left); serum starved and treated with l phosphatase (10,000 U/mL, 2 hr) ) (center, right); and 293 shRNA 4E-BP1/2 KO treated with U0126 #9903 (10 μM, 2 hr), LY294002 #9901 (50 μM, 2 hr), and Rapamycin #9904 (10 nM, 2 hr) (far right), using Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution. |
