| 货号 | 12032S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:400) |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from various cell lines, starved overnight and untreated (-) or treated with either TPA #4174 (200 nM, 15 min; +), hEGF #8916 (100 ng/ml, 15 min; +), or hPDGF-BB #8912 (100 ng/ml, 15 min; +), using Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb.血清饥饿过夜然后不处理（-）或用TPA #4174 (200 nM, 15 min)及Human hEGF #8916 (100 ng/mL, 15 min)处理（+），或TPA #4174 (200 nM, 15 min)及hPDGF-BB #8912 (100 ng/ml, 15 min; +)处理的多种细胞提取物进行，使用Phospho-p90RSK (Ser380) (D5D8)Rabbit mAb进行western blot分析。 | |
Immunoprecipitation of p90RSK from HeLa cell extracts, serum starved overnight and untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Normal Rabbit IgG #2729 (lanes 5 and 6) or Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb (lanes 3 and 4). Lanes 1 and 2 are 10% input. Western blot analysis was performed using Phospho-p90RSK (Ser380) Antibody #9341.血清饥饿过夜后不处理(-)或使用TPA #4174 (200 nM, 15 min; +)处理的HeLa细胞提取物，使用Rabbit IgG #2729 (lanes 5和 6)或Phospho-p90RSK (Ser380) (D5D8) 兔mAb(lanes 3和 4)对 p90RSK进行免疫沉淀实验。Lanes1和2是10%上样量。Phospho-p90RSK (Ser380)抗体#9341用作western blot分析。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.未处理（蓝色）或经过TPA #4174（绿色）处理的Jurkat细胞，使用Phospho-p90RSK (Ser380) (D5D8)Rabbit mAb进行流式分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414作为二抗。 |
