| 货号 | 8828S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This antibody also recognizes endogenous levels of Smad3 protein when phosphorylated Ser422 only or at both Ser423 and Ser425. |
| 使用方法 | WB(1:1000) F (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 52, 60 |
Confocal immunofluorescent analysis of HaCaT cells, serum-starved (left), treated with hTGF-β3 #8425 (100 ng/ml, 30 min; center), or treated with hTGF-β3 and SB 431542 (10 μg/mL, 1 hr; right), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (green) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8693 (red). 血清饥饿（左）的HaCaT细胞，使用hTGF-β3 #8425 (100 ng/ml, 30 min; 中),或使用hTGF-β3和SB 431542 (10 μg/mL, 1 hr;右)处理，使用Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4)Rabbit mAb（绿色）和COX IV (3E11)Rabbit mAb（偶联Alexa Fluor®555偶联）#8693（红色）进行激光共聚焦免疫荧光分析。 | |
Flow cytometric analysis of HT-1080 cells, untreated (blue), treated with hTGF-β3 #8425 (green), or treated with hTGF-β3 and SB 431542 (red), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb. 未处理（蓝色），用hTGF-β3 #8425（绿色）处理或hTGF-β3和SB 431542（红色）处理的HT-1080细胞，使用Phospho-Smad2 (Ser465/467)/Smad3(Ser423/425) (D27F4)Rabbit mAb兔单抗进行流式分析。 | |
Western blot analysis of extracts from HaCaT cells, untreated (-) or treated with hTGF-β3 #8425 (+) in the absence or presence of the TGFR inhibitor SB 431542, using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (upper) or Smad2/3 (D7G7) XP® Rabbit mAb #8685 (lower). 未处理（-）或在TGFR 抑制剂 SB 431542存在或不存在情况下处理hTGF-β3 #8425（+）HaCaT细胞，使用Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4)Rabbit mAb（上）或Smad2/3 (D7G7) XP® Rabbit mAb #8685（下）对细胞提取物进行western blot分析。 |
