eIF4G2/p97 (D88B6) XP® Rabbit mAb【科瑞奥博】

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eIF4G2/p97 (D88B6) XP® Rabbit mAb

货号： 5169S 基本售价： 3860.0 元规格： 100ul

产品信息

概述
货号	5169S
反应种属	Human/Mouse/Rat/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	eIF4G2/p97 (D88B6) XP® Rabbit mAb detects endogenous levels of total eIF4G2/p97 protein.
使用方法	WB(1:1000) IP (1:50) F (1:400) IF-IC (1:100)

性能
供应商	CST
背景	The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5 m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).
存放说明	-20C
计算分子量	97

参考图片

Confocal immunofluorescent analysis of HeLa cells using eIF4G2/p97 (D88B6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用eIF4G2/p97 (D88B6) XP® Rabbit mAb (绿色)，共聚焦免疫荧光观察eIF4G2/p97蛋白在HeLa细胞定位。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5®#4084 (DNA 荧光染料)。

Western blot analysis of extracts from various cell types using eIF4G2/p97 (D88B6) XP® Rabbit mAb.

使用eIF4G2/p97 (D88B6) XP® Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系的提取物。

Flow cytometric analysis of HeLa cells using eIF4G2/p97 (D88B6) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

与Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)比较，使用eIF4G2/p97 (D88B6) XP® Rabbit mAb兔单抗 (蓝色)，流式细胞术分析HeLa细胞。