| 货号 | 8822S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse nanog protein. This antibody is expected to recognize nanog 1a and 1b isoforms. This antibody does not cross-react with human nanog. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:1000) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 29-42 |
Western blot analysis of extracts from F9 and murine iPS cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific). | |
Confocal immunofluorescent analysis of F9 (left) and NIH/3T3 (right) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 F9 cells and either 5 μl of Nanog (D2A3) XP® Rabbit mAb (Mouse Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Flow cytometric analysis of NIH/3T3 (blue) and F9 (green) cells using Nanog (D2A3) XP® Rabbit mAb (Mouse Specific). |
