| 货号 | 5678S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | Smad2/3 Antibody recognizes endogenous levels of total Smad2/3 protein and overexpressed Smad2/3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:100) ChIP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 52, 60 |
| 参考文献 | 1 . Heldin, C.H. et al. (1997) Nature 390, 465-71. 2 . Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94. 3 . Derynck, R. et al. (1998) Cell 95, 737-40. 4 . Massagué, J. (1998) Annu Rev Biochem 67, 753-91. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Wu, G. et al. (2000) Science 287, 92-7. 7 . Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7. 8 . Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69. |
Confocal immunofluorescent analysis of HT-1080 cells, serum-starved (left), treated with Human TGF-β1 #8915 (1 μg/mL for 30 min; center) or treated with Human TGF-β1 and SB43152 (10 μg/mL for 30 min; right), using Smad2/Smad3 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). 饥饿处理（左），经人TGF-β1 #8915 (1 μg/mL for 30 min; 中)或人 TGF-β1和SB43152 (10 μg/mL for 30 min; 右)后的HT-1080 细胞，使用Smad2/Smad3抗体进行激光共聚焦荧光分析。（绿色）。使用DY-554phalloidin（红色）标记肌动蛋白丝。 | |
Flow cytometric analysis of HT-1080 cells using Smad2/3 Antibody (blue) compared to a Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用Smad2/3抗体（蓝色）和(DA1E)IgG XP® 同型对照兔单抗#3900 （红色）对 HT-1080细胞进行流式细胞分析。 | |
Western blot analysis of extracts from ACHN and HeLa cells using Smad2/3 Antibody. 使用Smad2/3抗体对ACHN和HeLa细胞提取物进行western blot分析。 | |
