| 货号 | 2266S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | This antibody detects endogenous levels of total PU.1 protein. The antibody does not cross react with other Ets family members. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) F (1:50) IF-IC (1:100) ChIP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3 enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).P |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Lloberas, J. et al. (1999) Immunol Today 20, 184-9. 2 . Klemsz, M.J. et al. (1990) Cell 61, 113-24. 3 . Dahl, R. and Simon, M.C. (2003) Blood Cells Mol Dis 31, 229-33. 4 . DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-41. 5 . Pongubala, J.M. et al. (1993) Science 259, 1622-5. 6 . Wang, J.M. et al. (2003) Mol Cell Biol 23, 1896-909. 7 . Zhang, P. et al. (1999) Proc Natl Acad Sci U S A 96, 8705-10. 8 . Moreau-Gachelin, F. et al. (1988) Nature 331, 277-80. |
Flow cytometric analysis of RAW cells, using PU.1 antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究RAW细胞，所用抗体为PU.1 antibody (蓝色) 与非特异性的阴性对照抗体(红色)。 | |
Western blot analysis of extracts from RAW, p388D1, WEHI-3 and THP1 cells using #2266.Western免疫印迹。用PU.1 Antibody #2266 研究RAW, p388D1, WEHI-3 和THP1 细胞的细胞提取液。 | |
DAPI staining (left) and immunofluorescent staining (right) of paraformaldehyde-fixed RAW cells using #2266.DAPI染色(左图) 和 免疫荧光染色(右图) 多聚甲醛固定的RAW 细胞，所用抗体为PU.1 Antibody#2266. | |
Immunohistochemical analysis of paraffin-embedded human tonsil, showing nuclear localization, using PU.1 Antibody.免疫组织化学染色分析石蜡包埋人扁桃体组织。所用抗体为PU.1 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization in lymphocytes, using PU.1 Antibody.免疫组织化学染色分析石蜡包埋人乳腺癌组织，图示为细胞核在淋巴细胞定位。所用抗体为PU.1 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (infiltrating cells), using PU.1 Antibody in the presence of control peptide (left) or PU.1 Blocking Peptide #1036 (right).免疫组织化学染色分析石蜡包埋人乳腺癌组织。在对照多肽(左图) 或PU.1封闭多肽#1036 (右图)存在下所用抗体为PU.1 Antibody 。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 U-937 cells and either 20 μl of PU.1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CD11b promoter primers, human CD18 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。U-937细胞培养至4 x 106，将染色质交联到玻片上,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μl PU.1抗体或2 μl Normal Rabbit IgG #2729 。对富集的DNA做real-time PCR,所用引物为human CD11b promoter primers, human CD18 intron 1 primers和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 |
