| 货号 | 2139S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | LSD1 Antibody detects endogenous levels of total LSD1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:400) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of cell lysates from various cell lines, using LSD1 Antibody. 使用LSD1 Antibody，免疫印迹(Western blot)分析不同细胞中LSD1的蛋白水平。 | |
Flow cytometric analysis of untreated HeLa cells, using LSD1 Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用LSD1 Antibody (蓝色)标记，流式细胞仪分析未处理的HeLa细胞。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization, using LSD1 Antibody. 使用LSD1 Antibody，免疫组化分析人源肺癌组织石蜡切片，结果显示为细胞核定位。 | |
Immunohistochemical staining of paraffin-embedded human breast carcinoma, using LSD1 Antibody preincubated with control peptide (left) or antigen-specific peptide (right). 使用LSD1 Antibody，左图：control peptide， 右图： antigen-specific peptide，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunofluorescent analysis of HeLa cells, using LSD1 Antibody (right). Nuclei are stained with DAPI (left). 使用LSD1 Antibody (右图)，免疫荧光分析HeLa细胞。DAPI染细胞核(左图)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 U-937 cells and either 10 μl of LSD1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Human CD11b Promoter Primers #14271, human SPI-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
