| 货号 | 3978S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | LIN28A (A177) Antibody detects endogenous levels of LIN28A protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts from various cell lines, including mouse embryonic stem cells (mESCs), using LIN28A (A177) Antibody. 使用LIN28A (A177)抗体对多种细胞提取物，包括小鼠胚胎干细胞（mESCs）进行western blot分析。 | |
Flow cytometric analysis of NCCIT cells using LIN28A (A177) Antibody (blue) compared to a nonspecific negative control antibody (red). 使用LIN28A (A177)抗体（蓝色）对NCCIT细胞进行流式细胞分析，非特异性阴性照抗体（红色）。 | |
Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用LIN28A (A177) 抗体（绿色）对P19细胞进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 phalloidin (红色)标记。蓝色假色=DRAQ5® #4084 (DNA荧光染料). |
