| 货号 | 4875S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | HSP90 (E289) Antibody detects endogenous levels of total HSP90 protein. This antibody does not cross-react with other HSPs. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:50) |
| 供应商 | CST |
| 背景 | HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP and co-chaperone dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Flow cytometric analysis of HeLa cells, using HSP90 (E289) Antibody (blue) compared to a nonspecific negative control antibody (red). 使用HSP90 (E289) Antibody（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞术分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP90 (E289) Antibody. 对石蜡包埋的人肺癌使用HSP90 (E289) Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP90 (E289) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人乳腺癌使用HSP90 (E289) Antibody进行免疫组织化学分析，其中左图使用对照肽，右图使用抗原特异性肽。 | |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using HSP90 (E289) Antibody. 对HeLa、NIH/3T3和C6细胞裂解液使用HSP90 (E289) Antibody进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP90 (E289) Antibody. 对石蜡包埋的人乳腺癌使用HSP90 (E289) Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP90 (E289) Antibody. 对石蜡包埋的人结肠癌使用HSP90 (E289) Antibody进行免疫组化分析。 |
