| 货号 | 2616S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | HP1 alpha antibody detects endogenous levels of total HP1alpha protein. The antibody does not cross-react with HP1 beta or HP1 gamma proteins. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:200) F (1:100) IF-IC (1:200) ChIP (1:25) |
| 供应商 | CST |
| 背景 | Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation (12-14). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Flow cytometric analysis of untreated HeLa cells, using HP1alpha antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用HP1alpha antibody (blue)标记，流式细胞仪分析非处理的HeLa细胞。 | |
Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using HP1alpha antibody. 使用HP1alpha antibody，免疫印迹(Western blot)分析HeLa, NIH/3T3, C6和COS细胞中HP1alpha蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HP1alpha Antibody in the presence of control peptide (left) or HP1 alpha blocking peptide #1004 (right). 使用HP1alpha Antibody，其分别与control peptide (左图)或HP1 alpha blocking peptide #1004 (右图)孵育，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Confocal immunofluorescent image of HeLa cells labeled with HP1α antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用HP1α antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 20 µl of HP1α Antibody #2616 or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse MEST Intron 1 Primers #12870, SimpleChIP® Mouse MEST Promoter Primers #12928, mouse Intracisternal A-Particle (IAP) LTR promoter primers, and mouse primers specific to a non-genic, non-repetitive region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
