| 货号 | 4844S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | COX IV Antibody detects endogenous levels of total COX IV protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:2400) F (1:100) |
| 供应商 | CST |
| 背景 | Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control. |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from HeLa (human), C6 (rat) and NIH/3T3 (mouse) cell lines using COX IV Antibody. 人Hela细胞,大鼠C6细胞和小鼠NIH/3T3细胞的western blot实验，所用抗体为COX IV Antibody | |
Flow cytometric analysis of HeLa cells, using Cox IV Antibody (blue) compared to a nonspecifc negative control antibody (red). 流式细胞检测Hela细胞系，所用抗体为Cox IV Antibody (绿)，对照为非特异性阴性对照（红） | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization using COX IV Antibody. 免疫组化检测石蜡包埋的人前列腺癌细胞，显示Cox IV Antibody 与胞浆的共定位 | |
Immunohistochemical analysis of paraffin-embedded human adenoma of the large intestine, using COX IV Antibody in the presence of control peptide (left) or antigen-specific peptide #1034 (right). 免疫组化检测石蜡包埋的人大肠腺瘤组织， 所用抗体为COX IV Antibody ，对照肽段（左）和抗原特异性肽#1034(右) |
