| 货号 | 3574S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | CD19 Antibody detects endogenous levels of CD19 independent of its phosphorylated state. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of SDS extracts from untreated or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells and untreated or anti-mouse IgG treated (30 µg/ml for 2 minutes) A20 cells, using control CD19 Antibody.Western免疫印迹。用control CD19 Antibody 研究未经处理的和经anti-human IgM (12 µg/ml,2 min) 处理的Ramos和未经处理和经anti-mouse IgG(30 µg/ml ,2 min)处理的 A20细胞的SDS细胞提取液。 | |
Flow cytometric analysis of Jurkat T cells (blue) and Ramos B cells (green), using CD19 Antibody.流式细胞仪分析Jurkat T细胞 (蓝色) 和 Ramos B 细胞 (绿色)所用抗体为CD19 Antibody。 | |
Confocal immunofluorescent analysis of Ramos (+) (left) and Jurkat (-) (right) cells using CD19 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
