| 货号 | 4443S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rat IgG1 |
| 应用 | W/IP/F |
| 目标/特异性 | CD3ε (CD3-12) Rat mAb detects endogenous levels of CD3ε protein. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) |
| 供应商 | CST |
| 背景 | When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck (5). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
Western blot analysis of total cell lysates from DND41 and Molt4 cells using CD3ε (CD3-12) Rat mAb.Western免疫印迹。用CD3ε (CD3-12) Rat mAb研究DND41和Molt4 细胞的细胞提取液。 | |
Flow cytometric analysis of Jurkat cells using CD3ε (CD3-12) Rat mAb Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析Jurkat细胞，所用抗体为CD3ε (CD3-12) Rat mAb Antibody (蓝色) 与非特异性对照抗体对比(红色)。 |
